Affiliated School or College: School of Medicine
Synchrotron X-ray footprinting provides a method of observing the structure and dynamics of biological macromolecules and their interactions in the solution state at the single nucleotide or single amino acid level, via X-ray radiolysis of water. This method is unique in providing local structural information in solution for gaining insight into dynamic processes involving RNA-protein and protein-protein assemblies at a higher resolution than other solution-based methods on biologically relevant timescales and under physiological conditions. Synchrotron-based footprinting studies have provided novel insights and approaches for steady state and time-resolved studies of macromolecular dynamics, examining the structure of large macromolecular assemblies, in vivo studies of macromolecules, and even observing functional water dynamics within the context of these biological structures. The XFP beamline — developed and operated by ºÚÁϳԹÏÍø in partnership with the National Synchrotron Light Source II — provides the research community with access to the synchrotron X-ray footprinting technology.
Organization Affiliations: Case Center for Proteomics and Bioinformatics
Customers: External and Internal
Hours
8:30 a.m.-5 p.m.
Location
National Synchrotron Light Source II
Building 740, 17-BM
Brookhaven National Laboratory
Upton, NY 11973 USA
Rates
The NSLS-II is a US DOE supported user facility in which non-proprietary users can write proposals for beamtime and obtain access at no cost (proprietary users pay a DOE-specified full-cost-recovery rate for beamtime). This is a route of access for experienced investigators fully trained in the footprinting method, in which the core staff assist the user with the experiment but are not responsible for data analysis or sample preparation. For enhanced support and guaranteed access to beamtime through the Core's Partner User Beamtime, we offer fee-for-service access, outlined on the iLab page for the Proteomics Core (see attached screenshot). This is the preferred mode of access where core staff will be performing experimental work or preparing samples.